CopA

• Description: copper-transporting ATPase, resistance to copper
  
  

Gene name	copA
Synonyms	yvgX
Essential	no
Product	copper transporting ATPase
Function	copper export, detoxification
Gene expression levels in SubtiExpress: copA
Interactions involving this protein in SubtInteract: CopA
Metabolic function and regulation of this protein in SubtiPathways: metal ion homeostasis
MW, pI	85 kDa, 5.484
Gene length, protein length	2409 bp, 803 aa
Immediate neighbours	cadA, copZ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, trace metal homeostasis (Cu, Zn, Ni, Mn, Mo), resistance against toxic metals, membrane proteins

This gene is a member of the following regulons

CsoR regulon

The gene

Basic information

• Locus tag: BSU33500

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + H₂O + Cu¹⁺(In) = ADP + phosphate + Cu¹⁺(Out) (according to Swiss-Prot)

• Protein family: Type IB subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • two N-terminal soluble domains, CopAa and CopAb, connected by a short linker

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • CopA-CopZ, for the transfer of the Cu(I) cluster PubMed

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:
  • 2RML ( N-terminal soluble domain)
  • 4BBJ (the protein from Legionella pneumophila, 45% identity, 79% similarity) PubMed

• UniProt: O32220

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: copZ-copA PubMed

• Expression browser: copA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by copper (CsoR) PubMed

• Regulatory mechanism:
  • CsoR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage

References

Reviews

Amy C Rosenzweig, José M Argüello
Toward a molecular understanding of metal transport by P(1B)-type ATPases.
Curr Top Membr: 2012, 69;113-36
[PubMed:23046649] [WorldCat.org] [DOI] (I p)

Amie K Boal, Amy C Rosenzweig
Structural biology of copper trafficking.
Chem Rev: 2009, 109(10);4760-79
[PubMed:19824702] [WorldCat.org] [DOI] (I p)