SpoIISA

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  • Description: lethal when synthesized during vegetative growth in the absence of SpoIISB

Gene name spoIISA
Synonyms ykaC
Essential no
Product toxin
Function programmed cell death
Gene expression levels in SubtiExpress: spoIISA
Interactions involving this protein in SubtInteract: SpoIISA
MW, pI 28 kDa, 4.683
Gene length, protein length 744 bp, 248 aa
Immediate neighbours spoIISB, pit
Sequences Protein DNA DNA_with_flanks
Genetic context
SpoIISA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SpoIISA expression.png
























Categories containing this gene/protein

sporulation proteins, toxins, antitoxins and immunity against toxins, membrane proteins

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

  • Locus tag: BSU12830

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: three putative membrane-spanning segments and a cytoplasmic domain
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • levels increase at the point of entry into stationary phase of cell cultures grown in sporulation-inducing medium, this is independent of Spo0A PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Patrik Florek, Vladimir M Levdikov, Elena Blagova, Andrey A Lebedev, Rostislav Škrabana, Stanislava Resetárová, Pamela Pavelcíková, Imrich Barak, Anthony J Wilkinson
The structure and interactions of SpoIISA and SpoIISB, a toxin-antitoxin system in Bacillus subtilis.
J Biol Chem: 2011, 286(8);6808-19
[PubMed:21147767] [WorldCat.org] [DOI] (I p)

Patrik Florek, Katarína Muchová, Pamela Pavelcíková, Imrich Barák
Expression of functional Bacillus SpoIISAB toxin-antitoxin modules in Escherichia coli.
FEMS Microbiol Lett: 2008, 278(2);177-84
[PubMed:18096016] [WorldCat.org] [DOI] (P p)

E Adler, I Barák, P Stragier
Bacillus subtilis locus encoding a killer protein and its antidote.
J Bacteriol: 2001, 183(12);3574-81
[PubMed:11371520] [WorldCat.org] [DOI] (P p)

Additional Publications: PubMed