OpuAC

• Description: glycine betaine ABC transporter (binding protein)
  
  

Gene name	opuAC
Synonyms
Essential	no
Product	glycine betaine ABC transporter (binding protein)
Function	compatible solute transport
Gene expression levels in SubtiExpress: opuAC
Interactions involving this protein in SubtInteract: OpuAC
Metabolic function and regulation of this protein in SubtiPathways: opuAC
MW, pI	32 kDa, 8.015
Gene length, protein length	879 bp, 293 aa
Immediate neighbours	opuAB, amhX
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, coping with hyper-osmotic stress, membrane proteins

This gene is a member of the following regulons

RemA regulon

The gene

Basic information

• Locus tag: BSU03000

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • binding for subsequent uptake of glycine betaine PubMed
  • binding for subsequent uptake of dimethylglycine PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:
  • K_D for glycine betaine: 26 µM PubMed
  • K_D for dimethylglycine: 172 µM PubMed

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • OpuAA-OpuAB-OpuAC PubMed

• Localization: associated to the membrane (via OpuAB) PubMed

Database entries

• Structure: 2B4L (complex with glycine betaine), 3CHG (in complex with DMSA)

• UniProt: P46922

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: opuAA-opuAB-opuAC PubMed

• Expression browser: opuAC PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by osmotic stress PubMed
  • repressed by intracellular glycine betaine PubMed

• Regulatory mechanism:
  • RemA: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Erhard Bremer, University of Marburg, Germany homepage