IspD

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  • Description: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, third step in the MEP pathway of isoprenoid biosynthesis

Gene name ispD
Synonyms yacM
Essential yes PubMed
Product 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Function MEP pathway of isoprenoid biosynthesis
Gene expression levels in SubtiExpress: ispD
MW, pI 25 kDa, 5.579
Gene length, protein length 696 bp, 232 aa
Immediate neighbours yacL, ispF
Sequences Protein DNA DNA_with_flanks
Genetic context
YacM context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
IspD expression.png















Categories containing this gene/protein

biosynthesis of lipids, general stress proteins (controlled by SigB), cell envelope stress proteins (controlled by SigM, V, W, X, Y), essential genes

This gene is a member of the following regulons

SigB regulon, SigM regulon

The gene

Basic information

  • Locus tag: BSU00900

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol (according to Swiss-Prot)
  • Protein family: ispD family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2YC3 (from Arabidopsis thaliana, 38% identity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References