YwaD

• Description: double-zinc aminopeptidase
  
  

Gene name	ywaD
Synonyms	ipa-8r
Essential	no
Product	double-zinc aminopeptidase
Function	unknown
Gene expression levels in SubtiExpress: ywaD
MW, pI	49 kDa, 6.638
Gene length, protein length	1365 bp, 455 aa
Immediate neighbours	tyrZ, ywaC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

poorly characterized/ putative enzymes

The gene

Basic information

• Locus tag: BSU38470

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: M28B subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • extracellular (signal peptide) PubMed

Database entries

• Structure:

• UniProt: P25152

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ywaD PubMed

• Expression browser: ywaD PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Xinxing Gao, Wenjing Cui, Ning Ding, Zhongmei Liu, Yaping Tian, Zhemin Zhou
Structure-based approach to alter the substrate specificity of Bacillus subtilis aminopeptidase.
Prion: 2013, 7(4);328-34
[PubMed:23787698] [WorldCat.org] [DOI] (I p)

Xinxing Gao, Wenjing Cui, Yaping Tian, Zhemin Zhou
Over-expression, secretion, biochemical characterisation, and structure analysis of Bacillus subtilis aminopeptidase.
J Sci Food Agric: 2013, 93(11);2810-5
[PubMed:23426795] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Yifat Fundoiano-Hershcovitz, Larisa Rabinovitch, Smadar Shulami, Vera Reiland, Gil Shoham, Yuval Shoham
The ywad gene from Bacillus subtilis encodes a double-zinc aminopeptidase.
FEMS Microbiol Lett: 2005, 243(1);157-63
[PubMed:15668014] [WorldCat.org] [DOI] (P p)