PucK

• Description: uric acid permease
  
  

Gene name	pucK
Synonyms	yunK
Essential	no
Product	uric acid permease
Function	purine utilization
Gene expression levels in SubtiExpress: pucK
Metabolic function and regulation of this protein in SubtiPathways: Purine catabolism, Nucleotides (regulation)
MW, pI	44 kDa, 8.348
Gene length, protein length	1290 bp, 430 aa
Immediate neighbours	pucJ, pucL
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, utilization of nucleotides, membrane proteins

This gene is a member of the following regulons

PucR regulon, TnrA regulon

The gene

Basic information

• Locus tag: BSU32440

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: xanthine/uracil permease family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O32140

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:
  • pucR-pucJ-pucK-pucL-pucM PubMed
  • pucJ-pucK-pucL-pucM PubMed

• Expression browser: pucK PubMed

• Sigma factor:
  • pucJ: SigA PubMed

• Regulation:
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed
  • PucR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: