YhdY

• Description: mechanosensitive channel, similar to MscS
  
  

Gene name	yhdY
Synonyms
Essential	no
Product	mechanosensitive channel, similar to MscS
Function	resistance to osmotic downshock
Gene expression levels in SubtiExpress: yhdY
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	42 kDa, 5.758
Gene length, protein length	1113 bp, 371 aa
Immediate neighbours	yhdX, srtN
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

coping with hypo-osmotic stress, membrane proteins

The gene

Basic information

• Locus tag: BSU09640

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O07594

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yhdY PubMed

• Expression browser: yhdY PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

• Mutant:
  • 1A958 ( yhdY::erm), PubMed, available at BGSC
  • 1A958 ( yhdY::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Erhard Bremer, University of Marburg, Germany homepage

References

Original Publications

Martin Lehnik-Habrink, Marc Schaffer, Ulrike Mäder, Christine Diethmaier, Christina Herzberg, Jörg Stülke
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y.
Mol Microbiol: 2011, 81(6);1459-73
[PubMed:21815947] [WorldCat.org] [DOI] (I p)

Paul G Wahome, Ann E Cowan, Barbara Setlow, Peter Setlow
Levels and localization of mechanosensitive channel proteins in Bacillus subtilis.
Arch Microbiol: 2009, 191(5);403-14
[PubMed:19252899] [WorldCat.org] [DOI] (I p)

Tamara Hoffmann, Clara Boiangiu, Susanne Moses, Erhard Bremer
Responses of Bacillus subtilis to hypotonic challenges: physiological contributions of mechanosensitive channels to cellular survival.
Appl Environ Microbiol: 2008, 74(8);2454-60
[PubMed:18310427] [WorldCat.org] [DOI] (I p)

Paul G Wahome, Peter Setlow
Growth, osmotic downshock resistance and differentiation of Bacillus subtilis strains lacking mechanosensitive channels.
Arch Microbiol: 2008, 189(1);49-58
[PubMed:17665170] [WorldCat.org] [DOI] (P p)