TreA

• Description: phospho-alpha-glucosidase
  
  

Gene name	treA
Synonyms	treC
Essential	no
Product	phospho-alpha-glucosidase
Function	trehalose utilization
Gene expression levels in SubtiExpress: treA
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	65 kDa, 4.895
Gene length, protein length	1683 bp, 561 aa
Immediate neighbours	treP, treR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, PhoP regulon, TreR regulon

The gene

Basic information

• Locus tag: BSU07810

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Alpha,alpha-trehalose 6-phosphate + H₂O = D-glucose + D-glucose 6-phosphate (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 13 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P39795

• KEGG entry: [3]

• E.C. number: 3.2.1.93

Expression and regulation

• Operon: treP-treA-treR PubMed

• Expression browser: treA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by trehalose (TreR) PubMed
  • repressed by glucose (CcpA) PubMed
  • expressed under conditions of phosphate limitation (PhoP) PubMed

• Regulatory mechanism:
  • TreR: transcription repression PubMed
  • CcpA: transcription repression PubMed
  • PhoP: transcription activation PubMed

• Additional information:

Biological materials

• Mutant: GP1118 (spc), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Hiromi Ujiie, Tomoko Matsutani, Hisashi Tomatsu, Ai Fujihara, Chisato Ushida, Yasuhiko Miwa, Yasutaro Fujita, Hyouta Himeno, Akira Muto
Trans-translation is involved in the CcpA-dependent tagging and degradation of TreP in Bacillus subtilis.
J Biochem: 2009, 145(1);59-66
[PubMed:18977770] [WorldCat.org] [DOI] (I p)

F Schöck, M K Dahl
Analysis of DNA flanking the treA gene of Bacillus subtilis reveals genes encoding a putative specific enzyme IITre and a potential regulator of the trehalose operon.
Gene: 1996, 175(1-2);59-63
[PubMed:8917076] [WorldCat.org] [DOI] (P p)

F Schöck, M K Dahl
Expression of the tre operon of Bacillus subtilis 168 is regulated by the repressor TreR.
J Bacteriol: 1996, 178(15);4576-81
[PubMed:8755887] [WorldCat.org] [DOI] (P p)

S Gotsche, M K Dahl
Purification and characterization of the phospho-alpha(1,1)glucosidase (TreA) of Bacillus subtilis 168.
J Bacteriol: 1995, 177(10);2721-6
[PubMed:7751281] [WorldCat.org] [DOI] (P p)

C Helfert, S Gotsche, M K Dahl
Cleavage of trehalose-phosphate in Bacillus subtilis is catalysed by a phospho-alpha-(1-1)-glucosidase encoded by the treA gene.
Mol Microbiol: 1995, 16(1);111-120
[PubMed:7651129] [WorldCat.org] [DOI] (P p)