TruB

• Description: tRNA pseudouridine 5S synthase
  
  

Gene name	truB
Synonyms	ylxN, ymxF
Essential	no
Product	tRNA pseudouridine 5S synthase
Function	tRNA modification
Gene expression levels in SubtiExpress: truB
MW, pI	34 kDa, 6.648
Gene length, protein length	927 bp, 309 aa
Immediate neighbours	rbfA, ribC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

The gene

Basic information

• Locus tag: BSU16660

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: tRNA uridine = tRNA pseudouridine (according to Swiss-Prot)

• Protein family: Type 1 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P32732

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: truB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jenna Friedt, Fern M V Leavens, Evan Mercier, Hans-Joachim Wieden, Ute Kothe
An arginine-aspartate network in the active site of bacterial TruB is critical for catalyzing pseudouridine formation.
Nucleic Acids Res: 2014, 42(6);3857-70
[PubMed:24371284] [WorldCat.org] [DOI] (I p)