XlyB

• Description: N-acetylmuramoyl-L-alanine amidase
  
  

Gene name	xlyB
Synonyms	yjpB
Essential	no
Product	N-acetylmuramoyl-L-alanine amidase
Function	PBSX prophage-mediated lysis
Gene expression levels in SubtiExpress: xlyB
MW, pI	33 kDa, 9.802
Gene length, protein length	951 bp, 317 aa
Immediate neighbours	yjpA, yjqA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover, phage-related functions, PBSX prophage

The gene

Basic information

• Locus tag: BSU12460

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)

• Protein family: LysM repeat (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • contains four N-acetylglucosamine-polymer-binding LysM domains PubMed
  • contains an amidase_2 domain (like BlyA, CwlA, CwlH, XlyB)

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • secreted (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O34391

• KEGG entry: [2]

• E.C. number: 3.5.1.28

Expression and regulation

• Operon:

• Expression browser: xlyB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Girbe Buist, Anton Steen, Jan Kok, Oscar P Kuipers
LysM, a widely distributed protein motif for binding to (peptido)glycans.
Mol Microbiol: 2008, 68(4);838-47
[PubMed:18430080] [WorldCat.org] [DOI] (I p)

Original publications

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)