TilS

• Description: trigger enzyme, tRNAIle-lysidine synthetase (modifies C34 at the wobble position of tRNA2Ile to lysidine) and part of a transcription activator
  
  

Gene name	tilS
Synonyms	yacA
Essential	yes PubMed
Product	tRNAIle-lysidine synthetase
Function	tRNA modification, control of ftsH expression
Gene expression levels in SubtiExpress: tilS
Interactions involving this protein in SubtInteract: TilS
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation)
MW, pI	53 kDa, 8.317
Gene length, protein length	1416 bp, 472 aa
Immediate neighbours	yabT, hprT
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, transcription factors and their control, cell envelope stress proteins (controlled by SigM, V, W, X, Y), essential genes

This gene is a member of the following regulons

SigM regulon

The TilS-HprT regulon

• ftsH

The gene

Basic information

• Locus tag: BSU00670

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • modifies C34 at the wobble position of tRNA2Ile to lysidine

• Protein family: tRNA(Ile)-lysidine synthase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • TilS-HprT, the complex activates transcription of ftsH PubMed

• Localization:
  • Cytoplasm (Homogeneous) PubMed

Database entries

• Structure: 3A2K (from Geobacillus kaustophilus, complex with tRNA) PubMed

• UniProt: P37563

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: tilS-hprT-ftsH PubMed

• Expression browser: tilS PubMed

• Sigma factor: SigM PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original publications

Caroline Köhrer, Debabrata Mandal, Kirk W Gaston, Henri Grosjean, Patrick A Limbach, Uttam L Rajbhandary
Life without tRNAIle-lysidine synthetase: translation of the isoleucine codon AUA in Bacillus subtilis lacking the canonical tRNA2Ile.
Nucleic Acids Res: 2014, 42(3);1904-15
[PubMed:24194599] [WorldCat.org] [DOI] (I p)

Ta-Hui Lin, Yi-Nei Hu, Gwo-Chyuan Shaw
Two enzymes, TilS and HprT, can form a complex to function as a transcriptional activator for the cell division protease gene ftsH in Bacillus subtilis.
J Biochem: 2014, 155(1);5-16
[PubMed:24001521] [WorldCat.org] [DOI] (I p)

Céline Fabret, Etienne Dervyn, Bérengère Dalmais, Alain Guillot, Christian Marck, Henri Grosjean, Philippe Noirot
Life without the essential bacterial tRNA Ile2-lysidine synthetase TilS: a case of tRNA gene recruitment in Bacillus subtilis.
Mol Microbiol: 2011, 80(4);1062-74
[PubMed:21435031] [WorldCat.org] [DOI] (I p)

Kotaro Nakanishi, Luc Bonnefond, Satoshi Kimura, Tsutomu Suzuki, Ryuichiro Ishitani, Osamu Nureki
Structural basis for translational fidelity ensured by transfer RNA lysidine synthetase.
Nature: 2009, 461(7267);1144-8
[PubMed:19847269] [WorldCat.org] [DOI] (I p)

Scott P Salowe, Judyann Wiltsie, Julio C Hawkins, Lisa M Sonatore
The catalytic flexibility of tRNAIle-lysidine synthetase can generate alternative tRNA substrates for isoleucyl-tRNA synthetase.
J Biol Chem: 2009, 284(15);9656-62
[PubMed:19233850] [WorldCat.org] [DOI] (P p)

Warawan Eiamphungporn, John D Helmann
The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.
Mol Microbiol: 2008, 67(4);830-48
[PubMed:18179421] [WorldCat.org] [DOI] (P p)

Alison Hunt, Joy P Rawlins, Helena B Thomaides, Jeff Errington
Functional analysis of 11 putative essential genes in Bacillus subtilis.
Microbiology (Reading): 2006, 152(Pt 10);2895-2907
[PubMed:17005971] [WorldCat.org] [DOI] (P p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

Akiko Soma, Yoshiho Ikeuchi, Satoru Kanemasa, Kazuo Kobayashi, Naotake Ogasawara, Tomotake Ote, Jun-ichi Kato, Kimitsuna Watanabe, Yasuhiko Sekine, Tsutomu Suzuki
An RNA-modifying enzyme that governs both the codon and amino acid specificities of isoleucine tRNA.
Mol Cell: 2003, 12(3);689-98
[PubMed:14527414] [WorldCat.org] [DOI] (P p)