FusA

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Revision as of 11:29, 20 August 2013 by Jstuelk (talk | contribs) (Extended information on the protein)
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  • Description: elongation factor G, facilitates movement of tRNA–mRNA by one codon

Gene name fusA
Synonyms fus
Essential yes PubMed
Product elongation factor G
Function translation
Gene expression levels in SubtiExpress: fusA
Interactions involving this protein in SubtInteract: FusA
MW, pI 76 kDa, 4.615
Gene length, protein length 2076 bp, 692 aa
Immediate neighbours rpsG, tufA
Sequences Protein DNA DNA_with_flanks
Genetic context
FusA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FusA expression.png















Categories containing this gene/protein

translation, essential genes, phosphoproteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

  • Locus tag: BSU01120

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: hydrolyses GTP
  • Protein family: EF-G/EF-2 subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Ser-213 AND Ser-302 AND Ser-569 AND Ser-680 AND (Thr-24 OR Thr-25) AND (Thr-43 OR Ser 48) PubMed, PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP840, available in Jörg Stülke's lab
    • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP848, available in Jörg Stülke's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References