SacB

• Description: levansucrase
  
  

Gene name	sacB
Synonyms
Essential	no
Product	levansucrase
Function	utilization of sucrose, production of levan
Gene expression levels in SubtiExpress: sacB
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism, Stress
MW, pI	52 kDa, 6.175
Gene length, protein length	1419 bp, 473 aa
Immediate neighbours	pbpE, levB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

DegU regulon, SacY regulon

The gene

Basic information

• Locus tag: BSU34450

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 68 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed

Database entries

• Structure: 1PT2 (complex with sucrose), 1OYG

• UniProt: P05655

• KEGG entry: [3]

• E.C. number: 2.4.1.10

Expression and regulation

• Operon: sacB-levB-yveA PubMed

• Expression browser: sacB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induction by sucrose (at high concentration) (SacY) PubMed

• Regulatory mechanism:
  • SacY: binding to a RNA switch (transcriptional antitermination) PubMed
  • DegU-P: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion: pGP564 (in pAC7), a series of RAT mutations in pAC7, all available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)