MsrA

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  • Description: peptide methionine sulfoxide reductase

Gene name msrA
Synonyms yppP
Essential no
Product peptide methionine sulfoxide reductase
Function regeneration of methionine and restoration
of protein function after oxidative damage
Gene expression levels in SubtiExpress: msrA
MW, pI 20 kDa, 5.709
Gene length, protein length 531 bp, 177 aa
Immediate neighbours msrB, ypoP
Sequences Protein DNA Advanced_DNA
Genetic context
MsrA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MsrA expression.png
























Categories containing this gene/protein

resistance against oxidative and electrophile stress

This gene is a member of the following regulons

Spx regulon

The gene

Basic information

  • Locus tag: BSU21690

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation: induced by oxidative stress (Spx)
  • Regulatory mechanism: Spx: oxidized Spx binds RpoA and enhances msrA-msrB operon transcription PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

CongHui You, Agnieszka Sekowska, Olivera Francetic, Isabelle Martin-Verstraete, YiPing Wang, Antoine Danchin
Spx mediates oxidative stress regulation of the methionine sulfoxide reductases operon in Bacillus subtilis.
BMC Microbiol: 2008, 8;128
[PubMed:18662407] [WorldCat.org] [DOI] (I e)