HypO

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  • Description: NAD(P)H-flavin nitroreductase

Gene name hypO
Synonyms yfkO
Essential no
Product NAD(P)H-flavin nitroreductase
Function protection against NaOCl stress
Gene expression levels in SubtiExpress: hypO
MW, pI 25 kDa, 5.667
Gene length, protein length 663 bp, 221 aa
Immediate neighbours treR, yfkN
Sequences Protein DNA Advanced_DNA
Genetic context
YfkO context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
HypO expression.png
























Categories containing this gene/protein

electron transport/ other, resistance against oxidative and electrophile stress

This gene is a member of the following regulons

HypR regulon

The gene

Basic information

  • Locus tag: BSU07830

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: nitroreductase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced under disulfide stress conditions (diamide, NaOCl) (HypR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Original articles

G A Prosser, A V Patterson, D F Ackerley
uvrB gene deletion enhances SOS chromotest sensitivity for nitroreductases that preferentially generate the 4-hydroxylamine metabolite of the anti-cancer prodrug CB1954.
J Biotechnol: 2010, 150(1);190-4
[PubMed:20727918] [WorldCat.org] [DOI] (I p)


Palm GJ, Khanh Chi B, Waack P, Gronau K, Becher D, Albrecht D, Hinrichs W, Read RJ, Antelmann H.
Structural insights into the redox-switch mechanism of the MarR/DUF24-type regulator HypR.
Nucleic Acids Res. 2012, Jan 11. [Epub ahead of print]
PubMed