GanB

• Description: endo-1,4-beta-galactosidase
  
  

Gene name	ganB
Synonyms	yvfO
Essential	no
Product	endo-1,4-beta-galactosidase
Function	galactan utilization
Gene expression levels in SubtiExpress: ganB
MW, pI	47 kDa, 8.243
Gene length, protein length	1287 bp, 429 aa
Immediate neighbours	rsbP, ganA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

LacR regulon

The gene

Basic information

• Locus tag: BSU34120

Phenotypes of a mutant

• a cycB-ganP-ganQ-ganA-ganB mutant is impaired in the utilization of galactan PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Endohydrolysis of (1->4)-beta-D-galactosidic linkages in galactan, generation of galactotriose PubMed

• Protein family: glycosyl hydrolase 53 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • likely to be extrecellular PubMed

Database entries

• Structure: 2J74 (B. licheniformis)

• UniProt: O07013

• KEGG entry: [3]

• E.C. number: 3.2.1.89

Expression and regulation

• Operon: cycB-ganP-ganQ-ganA-ganB PubMed

• Expression browser: ganB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • LacR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Yunrong Chai, Pascale B Beauregard, Hera Vlamakis, Richard Losick, Roberto Kolter
Galactose metabolism plays a crucial role in biofilm formation by Bacillus subtilis.
mBio: 2012, 3(4);e00184-12
[PubMed:22893383] [WorldCat.org] [DOI] (I e)

Stephanie Shipkowski, Jean E Brenchley
Bioinformatic, genetic, and biochemical evidence that some glycoside hydrolase family 42 beta-galactosidases are arabinogalactan type I oligomer hydrolases.
Appl Environ Microbiol: 2006, 72(12);7730-8
[PubMed:17056685] [WorldCat.org] [DOI] (P p)

R A Daniel, J Haiech, F Denizot, J Errington
Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR.
J Bacteriol: 1997, 179(17);5636-8
[PubMed:9287030] [WorldCat.org] [DOI] (P p)