MutTA

• Description: error prevention oxidized guanine system, confers protection against oxidative stress to vegetative cells, moreover the protein has RNA pyrophosphohydrolase activity
  
  

Gene name	mutTA
Synonyms	ytkD, rppH
Essential	no
Product	8-oxo-dGTPase (antimutator), RNA pyrophosphohydrolase
Function	DNA repair, RNA degradation
Gene expression levels in SubtiExpress: mutTA
MW, pI	18 kDa, 5.952
Gene length, protein length	474 bp, 158 aa
Immediate neighbours	ytlD, ytkC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins, RNases

This gene is a member of the following regulons

SigF regulon

The gene

Basic information

• Locus tag: BSU30630

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • removal of pyrophosphate from the 5' end of mRNAs to make the RNA accessible for degradation by RNases PubMed
  • RppH requires at least two unpaired nucleotides at the 5' end of its RNA substrates and prefers three or more. The second of these 5'-terminal nucleotides must be G, whereas a less strict preference for a purine is evident at the third position, and A is slightly favored over G at the first position PubMed

• Protein family: Nudix hydrolase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 4JZV PubMed

• UniProt: O35013

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mutTA PubMed

• Expression browser: mutTA PubMed

• Sigma factor: SigA PubMed, SigF PubMed

• Regulation:
  • expressed during logarithmic growth (SigA) and early during sporulation in the forespore (SigF) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Martin Lehnik-Habrink, Richard J Lewis, Ulrike Mäder, Jörg Stülke
RNA degradation in Bacillus subtilis: an interplay of essential endo- and exoribonucleases.
Mol Microbiol: 2012, 84(6);1005-17
[PubMed:22568516] [WorldCat.org] [DOI] (I p)

Original publications

Ping-kun Hsieh, Jamie Richards, Quansheng Liu, Joel G Belasco
Specificity of RppH-dependent RNA degradation in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2013, 110(22);8864-9
[PubMed:23610425] [WorldCat.org] [DOI] (I p)

Jérémie Piton, Valéry Larue, Yann Thillier, Audrey Dorléans, Olivier Pellegrini, Inés Li de la Sierra-Gallay, Jean-Jacques Vasseur, Françoise Debart, Carine Tisné, Ciarán Condon
Bacillus subtilis RNA deprotection enzyme RppH recognizes guanosine in the second position of its substrates.
Proc Natl Acad Sci U S A: 2013, 110(22);8858-63
[PubMed:23610407] [WorldCat.org] [DOI] (I p)

Jamie Richards, Quansheng Liu, Olivier Pellegrini, Helena Celesnik, Shiyi Yao, David H Bechhofer, Ciarán Condon, Joel G Belasco
An RNA pyrophosphohydrolase triggers 5'-exonucleolytic degradation of mRNA in Bacillus subtilis.
Mol Cell: 2011, 43(6);940-9
[PubMed:21925382] [WorldCat.org] [DOI] (I p)

Luz E Vidales, Lluvia C Cárdenas, Eduardo Robleto, Ronald E Yasbin, Mario Pedraza-Reyes
Defects in the error prevention oxidized guanine system potentiate stationary-phase mutagenesis in Bacillus subtilis.
J Bacteriol: 2009, 191(2);506-13
[PubMed:19011023] [WorldCat.org] [DOI] (I p)

Francisco X Castellanos-Juárez, Carlos Alvarez-Alvarez, Ronald E Yasbin, Barbara Setlow, Peter Setlow, Mario Pedraza-Reyes
YtkD and MutT protect vegetative cells but not spores of Bacillus subtilis from oxidative stress.
J Bacteriol: 2006, 188(6);2285-9
[PubMed:16513759] [WorldCat.org] [DOI] (P p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Wenlian Xu, Candice R Jones, Christopher A Dunn, Maurice J Bessman
Gene ytkD of Bacillus subtilis encodes an atypical nucleoside triphosphatase member of the Nudix hydrolase superfamily.
J Bacteriol: 2004, 186(24);8380-4
[PubMed:15576788] [WorldCat.org] [DOI] (P p)

Martha I Ramírez, Francisco X Castellanos-Juárez, Ronald E Yasbin, Mario Pedraza-Reyes
The ytkD (mutTA) gene of Bacillus subtilis encodes a functional antimutator 8-Oxo-(dGTP/GTP)ase and is under dual control of sigma A and sigma F RNA polymerases.
J Bacteriol: 2004, 186(4);1050-9
[PubMed:14761999] [WorldCat.org] [DOI] (P p)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)