LysA

• Description: diaminopimelate decarboxylase
  
  

Gene name	lysA
Synonyms
Essential	no
Product	diaminopimelate decarboxylase
Function	biosynthesis of lysine
Gene expression levels in SubtiExpress: lysA
Metabolic function and regulation of this protein in SubtiPathways: Lys, Thr
MW, pI	48 kDa, 5.007
Gene length, protein length	1317 bp, 439 aa
Immediate neighbours	ypuA, spoVAF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, sporulation proteins

This gene is a member of the following regulons

SigG regulon, SpoVT regulon

The gene

Basic information

• Locus tag: BSU23380

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Meso-2,6-diaminoheptanedioate = L-lysine + CO₂ (according to Swiss-Prot)

• Protein family: Orn/Lys/Arg decarboxylase class-II family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1HKW (from Mycobacterium tuberculosis, 42% identity, 61% similarity) PubMed

• UniProt: P23630

• KEGG entry: [3]

• E.C. number: 4.1.1.20

Expression and regulation

• Operon: spoVAA-spoVAB-spoVAC-spoVAD-spoVAEB-spoVAEA-spoVAF-lysA PubMed

• Expression browser: lysA PubMed

• Sigma factor: SigG PubMed

• Regulation:
  • expressed late during sporulation in the forespore (SigG, SpoVT) PubMed

• Regulatory mechanism:
  • SpoVT: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A615 ( lysA::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

I Bagyan, J Hobot, S Cutting
A compartmentalized regulator of developmental gene expression in Bacillus subtilis.
J Bacteriol: 1996, 178(15);4500-7
[PubMed:8755877] [WorldCat.org] [DOI] (P p)

V Azevedo, A Sorokin, S D Ehrlich, P Serror
The transcriptional organization of the Bacillus subtilis 168 chromosome region between the spoVAF and serA genetic loci.
Mol Microbiol: 1993, 10(2);397-405
[PubMed:7934830] [WorldCat.org] [DOI] (P p)

B Moldover, P J Piggot, M D Yudkin
Identification of the promoter and the transcriptional start site of the spoVA operon of Bacillus subtilis and Bacillus licheniformis.
J Gen Microbiol: 1991, 137(3);527-31
[PubMed:1903432] [WorldCat.org] [DOI] (P p)