NusA

• Description: transcription termination factor of RNA polymerase
  
  

Gene name	nusA
Synonyms
Essential	yes PubMed
Product	transcription termination factor
Function	transcription
Gene expression levels in SubtiExpress: nusA
Interactions involving this protein in SubtInteract: NusA
MW, pI	41 kDa, 4.583
Gene length, protein length	1113 bp, 371 aa
Immediate neighbours	ylxS, ylxR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU16600

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: nusA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • NusA-RpoB PubMed

• Localization:

Database entries

• Structure:

• UniProt: P32727

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ylxS-nusA-ylxR-ylxQ-infB-ylxP-rbfA PubMed

• Expression browser: nusA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Xiao Yang, Seeseei Molimau, Geoff P Doherty, Elecia B Johnston, Jon Marles-Wright, Rosalba Rothnagel, Ben Hankamer, Richard J Lewis, Peter J Lewis
The structure of bacterial RNA polymerase in complex with the essential transcription elongation factor NusA.
EMBO Rep: 2009, 10(9);997-1002
[PubMed:19680289] [WorldCat.org] [DOI] (I p)

Karen M Davies, Amy J Dedman, Stephanie van Horck, Peter J Lewis
The NusA:RNA polymerase ratio is increased at sites of rRNA synthesis in Bacillus subtilis.
Mol Microbiol: 2005, 57(2);366-79
[PubMed:15978071] [WorldCat.org] [DOI] (P p)

Alexander V Yakhnin, Paul Babitzke
NusA-stimulated RNA polymerase pausing and termination participates in the Bacillus subtilis trp operon attenuation mechanism invitro.
Proc Natl Acad Sci U S A: 2002, 99(17);11067-72
[PubMed:12161562] [WorldCat.org] [DOI] (P p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)

C J Ingham, J Dennis, P A Furneaux
Autogenous regulation of transcription termination factor Rho and the requirement for Nus factors in Bacillus subtilis.
Mol Microbiol: 1999, 31(2);651-63
[PubMed:10027981] [WorldCat.org] [DOI] (P p)

K Shazand, J Tucker, M Grunberg-Manago, J C Rabinowitz, T Leighton
Similar organization of the nusA-infB operon in Bacillus subtilis and Escherichia coli.
J Bacteriol: 1993, 175(10);2880-7
[PubMed:8491709] [WorldCat.org] [DOI] (P p)