Fmt

• Description: methionyl-tRNA formyltransferase
  
  

Gene name	fmt
Synonyms	yloL
Essential	yes PubMed
Product	methionyl-tRNA formyltransferase
Function	formylation of Met-tRNA(fMet)
Gene expression levels in SubtiExpress: fmt
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging
MW, pI	34 kDa, 5.618
Gene length, protein length	951 bp, 317 aa
Immediate neighbours	def, yloM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

Efp-dependent proteins

The gene

Basic information

• Locus tag: BSU15730

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) + H₂O = tetrahydrofolate + N-formylmethionyl-tRNA(fMet) (according to Swiss-Prot)

• Protein family: fmt family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3RFO (from Bacillus anthracis, 69% identity, 89% similarity)

• UniProt: P94463

• KEGG entry: [2]

• E.C. number: 2.1.2.9

Expression and regulation

• Operon: def-fmt-yloM PubMed

• Expression browser: fmt PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional references: PubMed