Rnc
- Description: RNase III; required for the degradation of sense/antisense transcripts in Gram positive, low GC bacteria
Gene name | rnc |
Synonyms | rncS |
Essential | yes |
Product | endoribonuclease III |
Function | cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr) |
Gene expression levels in SubtiExpress: rnc | |
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion | |
MW, pI | 28 kDa, 8.076 |
Gene length, protein length | 747 bp, 249 aa |
Immediate neighbours | acpA, smc |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
Rnases, translation, protein secretion, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU15930
Phenotypes of a mutant
essential PubMed
rnc is essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot) cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr)
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P51833
- KEGG entry: [3]
- E.C. number: 3.1.26.3
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
David Bechhofer, Mount Sinai School, New York, USA Homepage
Ciaran Condon, IBPC, Paris, France Homepage
Your additional remarks
References
Reviews
Original publications