SppA

• Description: signal peptide peptidase required for efficient processing of pre-proteins
  
  

Gene name	sppA
Synonyms	yteI
Essential	no
Product	signal peptide peptidase
Function	protein secretion
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	36 kDa, 7.314
Gene length, protein length	1005 bp, 335 aa
Immediate neighbours	yteJ, ytdI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein secretion, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

SigW regulon

The gene

Basic information

• Locus tag: BSU29530

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • serine protease that functions to cleave the remnant signal peptides left behind after protein secretion and cleavage by signal peptidases

• Protein family: peptidase S49 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • the protein forms a dome shaped octameric complex PubMed

• Localization:
  • cell membrane PubMed

Database entries

• Structure: 3RST PubMed

• UniProt: O34525

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sppA-yteJ PubMed

• Expression browser: sppA PubMed

• Sigma factor: SigW PubMed

• Regulation:
  • induced in response to cell wall stress (SigW) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Sung-Eun Nam, Apollos C Kim, Mark Paetzel
Crystal structure of Bacillus subtilis signal peptide peptidase A.
J Mol Biol: 2012, 419(5);347-58
[PubMed:22472423] [WorldCat.org] [DOI] (I p)

Min Cao, Tao Wang, Rick Ye, John D Helmann
Antibiotics that inhibit cell wall biosynthesis induce expression of the Bacillus subtilis sigma(W) and sigma(M) regulons.
Mol Microbiol: 2002, 45(5);1267-76
[PubMed:12207695] [WorldCat.org] [DOI] (P p)

X Huang, A Gaballa, M Cao, J D Helmann
Identification of target promoters for the Bacillus subtilis extracytoplasmic function sigma factor, sigma W.
Mol Microbiol: 1999, 31(1);361-71
[PubMed:9987136] [WorldCat.org] [DOI] (P p)