Rph

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  • Description: RNase PH, 3'-5' exoribonuclease

Gene name rph
Synonyms
Essential no
Product RNase PH (EC 2.7.7.56)
Function 3'-5' exoribonuclease
MW, pI 26 kDa, 4.888
Gene length, protein length 735 bp, 245 aa
Immediate neighbours ysnA, gerM
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Rph context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Rph expression.png
























Categories containing this gene/protein

Rnases, translation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU28370

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • major player in the exonucleolytic maturation of CCA-containing tRNA precursors PubMed
    • responsible for endonucleolytic cleavage of scRNA (scr) PubMed
  • Protein family: RNase PH family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage

Ciaran Condon, IBPC, Paris, France Homepage

Your additional remarks

References