YacF

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  • Description: putative tRNA-dihydrouridine synthase B

Gene name yacF
Synonyms dusB, dus1
Essential no
Product putative tRNA-dihydrouridine synthase B
Function tRNA maturation
Metabolic function and regulation of this protein in SubtiPathways:
Folate
MW, pI 36 kDa, 7.868
Gene length, protein length 999 bp, 333 aa
Immediate neighbours yazB, lysS
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YacF context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

translation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU00810

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: dus family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1VHN (from Thermotoga maritima, 35% identity, 54% similarity) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References