Cca

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  • Description: tRNA nucleotidyltransferase, maturation of the single-copy tRNACys, which lacks an encoded CCA 3' end

Gene name cca
Synonyms papS, ypjI
Essential yes PubMed
Product tRNA nucleotidyltransferase
Function tRNA modification
MW, pI 45 kDa, 8.041
Gene length, protein length 1191 bp, 397 aa
Immediate neighbours birA, bshA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Cca context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Cca expression.png
























Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU22450

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + tRNA(n) = diphosphate + tRNA(n+1) (according to Swiss-Prot)
  • Protein family: the protein is similar to the E. coli poly(A) polymerase
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1MIV (Geobacillus stearothermophilus 44% identity)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Heike Betat, Christiane Rammelt, Mario Mörl
tRNA nucleotidyltransferases: ancient catalysts with an unusual mechanism of polymerization.
Cell Mol Life Sci: 2010, 67(9);1447-63
[PubMed:20155482] [WorldCat.org] [DOI] (I p)

Stefan Vörtler, Mario Mörl
tRNA-nucleotidyltransferases: highly unusual RNA polymerases with vital functions.
FEBS Lett: 2010, 584(2);297-302
[PubMed:19883645] [WorldCat.org] [DOI] (I p)

Anne Neuenfeldt, Andrea Just, Heike Betat, Mario Mörl
Evolution of tRNA nucleotidyltransferases: a small deletion generated CC-adding enzymes.
Proc Natl Acad Sci U S A: 2008, 105(23);7953-8
[PubMed:18523015] [WorldCat.org] [DOI] (I p)

Yong Xiong, Thomas A Steitz
A story with a good ending: tRNA 3'-end maturation by CCA-adding enzymes.
Curr Opin Struct Biol: 2006, 16(1);12-7
[PubMed:16364630] [WorldCat.org] [DOI] (P p)

Original publications

Operon and expression