RnjB

• Description: RNase J2
  
  

Gene name	rnjB
Synonyms	ymfA
Essential	no
Product	RNase J2
Function	RNA processing and degradation
Interactions involving this protein in SubtInteract: RNase J2
MW, pI	56 kDa, 9.18
Gene length, protein length	1545 bp, 515 aa
Immediate neighbours	dapA, tepA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

Rnases

The gene

Basic information

• Locus tag: BSU16780

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: endoribonuclease, involved in processing of thrS mRNA

• Protein family: RNase J subfamily (according to Swiss-Prot)

• Paralogous protein(s): RnjA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • associated to the RNA degradosome PubMed
  • RnjA-RnjB PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 3ZQ4 (RNase J1) PubMed

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: rnjB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP45 (spc), GP1113 (miniTn10 spc), both available in Stülke lab

• Expression vector:

• lacZ fusion: pGP419 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• FLAG-tag construct: GP1001 (spc, based on pGP1331), available in the Stülke lab

• Antibody:

Labs working on this gene/protein

Harald Putzer, IBPC Paris, France Homepage

References

Reviews

Additional reviews: PubMed

Lehnik-Habrink M, Lewis RJ, Mäder U, Stülke J    
RNA degradation in Bacillus subtilis: an interplay of
essential endo- and exoribonucleases. 
Mol Microbiol.: 2012, 84(6) 1005-1017. 
PubMed:22568516

Jamie Richards, Joel G Belasco
Ribonuclease J: how to lead a double life.
Structure: 2011, 19(9);1201-3
[PubMed:21893280] [WorldCat.org] [DOI] (I p)

Ciarán Condon
What is the role of RNase J in mRNA turnover?
RNA Biol: 2010, 7(3);316-21
[PubMed:20458164] [WorldCat.org] [DOI] (I p)

Original publications

Additional publications: PubMed