ProB

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  • Description: glutamate 5-kinase

Gene name proB
Synonyms
Essential no
Product glutamate 5-kinase
Function biosynthesis of proline
Metabolic function and regulation of this protein in SubtiPathways:
Pro, Stress
MW, pI 39 kDa, 6.01
Gene length, protein length 1095 bp, 365 aa
Immediate neighbours ykkE, proA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ProB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ProB expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

T-box

The gene

Basic information

  • Locus tag: BSU13120

Phenotypes of a mutant

  • auxotrophic for proline PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-glutamate = ADP + L-glutamate 5-phosphate (according to Swiss-Prot)
  • Protein family: PUA domain (according to Swiss-Prot)
  • Paralogous protein(s): ProJ

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: the enzymatic activity is feedback-inhibited by proline {PubMed|16958849}}

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Jeanette Brill, Tamara Hoffmann, Harald Putzer, Erhard Bremer
T-box-mediated control of the anabolic proline biosynthetic genes of Bacillus subtilis.
Microbiology (Reading): 2011, 157(Pt 4);977-987
[PubMed:21233158] [WorldCat.org] [DOI] (I p)

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Mingqing Chen, Hongbo Wei, JunWei Cao, Ruijie Liu, Youliang Wang, Congyi Zheng
Expression of Bacillus subtilis proBA genes and reduction of feedback inhibition of proline synthesis increases proline production and confers osmotolerance in transgenic Arabidopsis.
J Biochem Mol Biol: 2007, 40(3);396-403
[PubMed:17562291] [WorldCat.org] [DOI] (P p)

Mingqing Chen, Junwei Cao, Congyi Zheng, Qing Liu
Directed evolution of an artificial bifunctional enzyme, gamma-glutamyl kinase/gamma-glutamyl phosphate reductase, for improved osmotic tolerance of Escherichia coli transformants.
FEMS Microbiol Lett: 2006, 263(1);41-7
[PubMed:16958849] [WorldCat.org] [DOI] (P p)