IlvB

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  • Description: write here

Gene name ilvB
Synonyms
Essential
Product acetolactate synthase (acetohydroxy-acid synthase) (large subunit) (EC 4.1.3.18)
Function
MW, pI 62 kDa, 5.178
Gene length, protein length 1722 bp, 574 aa
Immediate neighbours
Gene sequence (+200bp) Protein sequence
Genetic context
IlvB context.gif



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry:
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: expressed in the absence of branched-chain amino acids (BCAA), expression is stimulated in the presence of glucose (CcpA) PubMed, during growth repressed under nitrogen limiting conditions (TnrA) PubMed
  • Regulatory mechanism: glucose regulation: CcpA PubMed, TnrA: transcription repession PubMed, repression by BCAA: tRNA-controlled RNA switch (T-box) that mediates termination/antitermination, transcription repression by CodY
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Grandoni, J. A., S. A. Zahler, and J. M. Calvo. 1992. Transcriptional regulation of the ilv-leu operon of Bacillus subtilis. J. Bacteriol. 174:3212-3219. PubMed
  2. Ludwig, H., Meinken, C., Matin, A. & Stülke, J. (2002) Insufficient expression of the ilv-leu operon encoding enzymes of branched-chain amino acids biosynthesis limits growth of a Bacillus subtilis ccpA mutant. J. Bacteriol. 184: 5174-5178. PubMed
  3. Shivers RP, Sonenshein AL: Bacillus subtilis ilvB operon: an intersection of global regulons. Mol Microbiol 2005, 56:1549-1559. PubMed
  4. Tojo S, Satomura T, Morisaki K, Deutscher J, Hirooka K, Fujita Y: Elaborate transcription regulation of the Bacillus subtilis ilv-leu operon involved in the biosynthesis of branched-chain amino acids through global regulators of CcpA, CodY and TnrA. Mol Microbiol 2005, 56:1560-1573. PubMed
  5. Tojo S, Satomura T, Morisaki K, Yoshida KI, Hirooka K, Fujita Y: Negative transcriptional regulation of the ilv-leu operon for biosynthesis of branched-chain amino acids through the Bacillus subtilis global regulator TnrA. J Bacteriol 2004, 186:7971-7979. PubMed
  6. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed