CsrA

• Description: motility regulator, binds to the hag mRNA to inhibit its translation
  
  

Gene name	csrA
Synonyms	yviG
Essential	no
Product	motility regulator
Function	control of hag translation
Interactions involving this protein in SubtInteract: CsrA
MW, pI	8 kDa, 6.09
Gene length, protein length	222 bp, 74 aa
Immediate neighbours	hag, fliW
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

RNA binding regulators, motility and chemotaxis

The gene

Basic information

• Locus tag: BSU35370

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • CsrA binds the hag mRNA to inhibit translation PubMed

• Protein family: csrA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • CsrA is sequestered by interaction with FliW (the two proteins form a conserved module in many bacteria) PubMed

• Interactions:
  • CsrA-RsmY (hypothetical)
  • FliW-CsrA PubMed

• Localization:

Database entries

• Structure: 1T3O

• UniProt: P33911

• KEGG entry: [3]

• E.C. number:

Additional information

• CsrA and FliW form a conserved module in many bacteria PubMed

Expression and regulation

• Operon: csrA PubMed

• Expression browser: csrA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP469 (spc), available in Stülke lab

• Expression vector:
  • pGP381 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
  • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP383, available in Stülke lab

• lacZ fusion: pGP461 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Tony Romeo, Christopher A Vakulskas, Paul Babitzke
Post-transcriptional regulation on a global scale: form and function of Csr/Rsm systems.
Environ Microbiol: 2013, 15(2);313-24
[PubMed:22672726] [WorldCat.org] [DOI] (I p)

Paul Babitzke, Carol S Baker, Tony Romeo
Regulation of translation initiation by RNA binding proteins.
Annu Rev Microbiol: 2009, 63;27-44
[PubMed:19385727] [WorldCat.org] [DOI] (I p)