YxeI
- Description: penicillin acylase
| Gene name | yxeI |
| Synonyms | |
| Essential | no |
| Product | penicillin acylase |
| Function | resistance to penicillin |
| MW, pI | 37 kDa, 5.276 |
| Gene length, protein length | 984 bp, 328 aa |
| Immediate neighbours | yxeJ, yxeH |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
Resistance against toxins/ antibiotics
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU39540
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: acylation of penicillin V PubMed
- Protein family: peptidase C59 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- the apparent K(m) values for penicillin V and the synthetic substrate 2-nitro-5-(phenoxyacetamido)-benzoic acid was 40mM and 0.63mM, respectively PubMed
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- homotetramer PubMed
Database entries
- Structure: 2OQC
- UniProt: P54948
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Priya Rathinaswamy, Sushama M Gaikwad, C G Suresh, Asmita A Prabhune, James A Brannigan, Guy G Dodson, Archana V Pundle
Purification and characterization of YxeI, a penicillin acylase from Bacillus subtilis.
Int J Biol Macromol: 2012, 50(1);25-30
[PubMed:21978958]
[WorldCat.org]
[DOI]
(I p)
Kyle N Erwin, Shunji Nakano, Peter Zuber
Sulfate-dependent repression of genes that function in organosulfur metabolism in Bacillus subtilis requires Spx.
J Bacteriol: 2005, 187(12);4042-9
[PubMed:15937167]
[WorldCat.org]
[DOI]
(P p)
Ken-Ichi Yoshida, Izumi Ishio, Eishi Nagakawa, Yoshiyuki Yamamoto, Mami Yamamoto, Yasutaro Fujita
Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome.
Microbiology (Reading): 2000, 146 ( Pt 3);573-579
[PubMed:10746760]
[WorldCat.org]
[DOI]
(P p)
