SecDF

• Description: preprotein translocase subunit (ATPase)
  
  

Gene name	secDF
Synonyms	secF, secD
Essential	no
Product	preprotein translocase subunit (ATPase)
Function	protein secretion
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	81 kDa, 9.519
Gene length, protein length	2211 bp, 737 aa
Immediate neighbours	yrvC, comN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

protein secretion, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

• Locus tag: BSU27650

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • membrane PubMed

Database entries

• Structure:

• UniProt: O32047

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor: SigM PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • SM-GN3 (secDF-spc), available in Anne Galinier's and Boris Görke's labs

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Warawan Eiamphungporn, John D Helmann
The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.
Mol Microbiol: 2008, 67(4);830-48
[PubMed:18179421] [WorldCat.org] [DOI] (P p)

Aileen Rubio, Xin Jiang, Kit Pogliano
Localization of translocation complex components in Bacillus subtilis: enrichment of the signal recognition particle receptor at early sporulation septa.
J Bacteriol: 2005, 187(14);5000-2
[PubMed:15995216] [WorldCat.org] [DOI] (P p)