NusB

• Description: probable transcription termination
  
  

Gene name	nusB
Synonyms	yqhZ
Essential	no
Product	unknown
Function	unknown
MW, pI	14 kDa, 4.757
Gene length, protein length	393 bp, 131 aa
Immediate neighbours	folD, yqhY
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transcription

This gene is a member of the following regulons

PurR regulon, stringent response

The gene

Basic information

• Locus tag: BSU24320

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: nusB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P54520

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nusB-folD PubMed

• Sigma factor:

• Regulation:
  • repressed in the presence of purine nucleotides (PurR) PubMed
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:
  • PurR: transcription repression (molecular inducer: PRPP) PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Geoff P Doherty, Donna H Meredith, Peter J Lewis
Subcellular partitioning of transcription factors in Bacillus subtilis.
J Bacteriol: 2006, 188(11);4101-10
[PubMed:16707701] [WorldCat.org] [DOI] (P p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)

H H Saxild, K Brunstedt, K I Nielsen, H Jarmer, P Nygaard
Definition of the Bacillus subtilis PurR operator using genetic and bioinformatic tools and expansion of the PurR regulon with glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO.
J Bacteriol: 2001, 183(21);6175-83
[PubMed:11591660] [WorldCat.org] [DOI] (P p)

D J Ebbole, H Zalkin
Interaction of a putative repressor protein with an extended control region of the Bacillus subtilis pur operon.
J Biol Chem: 1989, 264(6);3553-61
[PubMed:2536750] [WorldCat.org] (P p)