Rnc

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  • Description: RNase III; required for the degradation of sense/antisense transcripts in Gram positive, low GC bacteria

Gene name rnc
Synonyms rncS
Essential yes
Product endoribonuclease III
Function cleaves both 5'- and 3'-sites
of the small cytoplasmic RNA precursor (scr)
Metabolic function and regulation of this protein in SubtiPathways:
Protein secretion
MW, pI 28 kDa, 8.076
Gene length, protein length 747 bp, 249 aa
Immediate neighbours acpA, smc
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Rnc context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

Rnases, translation, protein secretion, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

  • Locus tag: BSU15930

Phenotypes of a mutant

essential PubMed

rnc is essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot) cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number: 3.1.26.3

Additional information

Expression and regulation

  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage

Ciaran Condon, IBPC, Paris, France Homepage

Your additional remarks

References

Reviews

Ian J MacRae, Jennifer A Doudna
Ribonuclease revisited: structural insights into ribonuclease III family enzymes.
Curr Opin Struct Biol: 2007, 17(1);138-45
[PubMed:17194582] [WorldCat.org] [DOI] (P p)

Djamel Drider, Ciarán Condon
The continuing story of endoribonuclease III.
J Mol Microbiol Biotechnol: 2004, 8(4);195-200
[PubMed:16179796] [WorldCat.org] [DOI] (P p)

R A Srivastava, N Srivastava
The multifaceted roles of the RNA processing enzyme ribonuclease III.
Indian J Biochem Biophys: 1996, 33(4);253-60
[PubMed:8936814] [WorldCat.org] (P p)

Original publications