MtnN

• Description: methylthioadenosine nucleosidase
  
  

Gene name	mtnN
Synonyms	yrrU, mtn
Essential	no
Product	methylthioadenosine nucleosidase
Function	methionine salvage
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	25 kDa, 4.844
Gene length, protein length	693 bp, 231 aa
Immediate neighbours	mccA, yrrT
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

CymR regulon, Spx regulon

The gene

Basic information

• Locus tag: BSU27270

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: S-adenosyl-L-homocysteine + H₂O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine (according to Swiss-Prot)

• Protein family: MtnN subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1JYS (from Escherichia coli, 51% identity, 70% similarity) PubMed

• UniProt: O32028

• KEGG entry: [3]

• E.C. number: 3.2.2.9

Expression and regulation

• Operon: yrrT-mtnN-mccA-mccB-yrhC PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of cysteine (CymR) PubMed

• Regulatory mechanism:
  • CymR: transcription repression PubMed
  • Spx: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: