SspC

• Description: small acid-soluble spore protein (minor alpha/beta-type SASP)
  
  

Gene name	sspC
Synonyms
Essential	no
Product	small acid-soluble spore protein (minor alpha/beta-type SASP)
Function	protection of spore DNA
MW, pI	7 kDa, 9.102
Gene length, protein length	216 bp, 72 aa
Immediate neighbours	yotB, yosZ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

sporulation proteins, SP-beta prophage

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

• Locus tag: BSU19950

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2Z3X (in complex with DNA)

• UniProt: P02958

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sspC PubMed

• Sigma factor: SigG PubMed

• Regulation:
  • expressed late during sporulation in the forespore (SigG) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ki Seog Lee, Daniela Bumbaca, Jeffrey Kosman, Peter Setlow, Mark J Jedrzejas
Structure of a protein-DNA complex essential for DNA protection in spores of Bacillus species.
Proc Natl Acad Sci U S A: 2008, 105(8);2806-11
[PubMed:18287075] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Daphna Frenkiel-Krispin, Rinat Sack, Joseph Englander, Eyal Shimoni, Miriam Eisenstein, Esther Bullitt, Rachel Horowitz-Scherer, Christopher S Hayes, Peter Setlow, Abraham Minsky, Sharon Grayer Wolf
Structure of the DNA-SspC complex: implications for DNA packaging, protection, and repair in bacterial spores.
J Bacteriol: 2004, 186(11);3525-30
[PubMed:15150240] [WorldCat.org] [DOI] (P p)

Jeffrey Kosman, Peter Setlow
Effects of carboxy-terminal modifications and pH on binding of a Bacillus subtilis small, acid-soluble spore protein to DNA.
J Bacteriol: 2003, 185(20);6095-103
[PubMed:14526021] [WorldCat.org] [DOI] (P p)

Anjum Sohail, Christopher S Hayes, Pradeep Divvela, Peter Setlow, Ashok S Bhagwat
Protection of DNA by alpha/beta-type small, acid-soluble proteins from Bacillus subtilis spores against cytosine deamination.
Biochemistry: 2002, 41(38);11325-30
[PubMed:12234173] [WorldCat.org] [DOI] (P p)

C S Hayes, P Setlow
An alpha/beta-type, small, acid-soluble spore protein which has very high affinity for DNA prevents outgrowth of Bacillus subtilis spores.
J Bacteriol: 2001, 183(8);2662-6
[PubMed:11274127] [WorldCat.org] [DOI] (P p)

C S Hayes, E Alarcon-Hernandez, P Setlow
N-terminal amino acid residues mediate protein-protein interactions between DNA-bound alpha /beta -type small, acid-soluble spore proteins from Bacillus species.
J Biol Chem: 2001, 276(3);2267-75
[PubMed:11044450] [WorldCat.org] [DOI] (P p)

C S Hayes, B Illades-Aguiar, L Casillas-Martinez, P Setlow
In vitro and in vivo oxidation of methionine residues in small, acid-soluble spore proteins from Bacillus species.
J Bacteriol: 1998, 180(10);2694-700
[PubMed:9573155] [WorldCat.org] [DOI] (P p)

Y Carrillo-Martinez, P Setlow
Properties of Bacillus subtilis small, acid-soluble spore proteins with changes in the sequence recognized by their specific protease.
J Bacteriol: 1994, 176(17);5357-63
[PubMed:8071212] [WorldCat.org] [DOI] (P p)

W L Nicholson, D X Sun, B Setlow, P Setlow
Promoter specificity of sigma G-containing RNA polymerase from sporulating cells of Bacillus subtilis: identification of a group of forespore-specific promoters.
J Bacteriol: 1989, 171(5);2708-18
[PubMed:2468649] [WorldCat.org] [DOI] (P p)

M J Connors, P Setlow
Cloning of a small, acid-soluble spore protein gene from Bacillus subtilis and determination of its complete nucleotide sequence.
J Bacteriol: 1985, 161(1);333-9
[PubMed:2981806] [WorldCat.org] [DOI] (P p)