SpoIISA

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  • Description: lethal when synthesized during vegetative growth in the absence of SpoIISB

Gene name spoIISA
Synonyms ykaC
Essential no
Product toxin
Function programmed cell death
Interactions involving this protein in SubtInteract: SpoIISA
MW, pI 28 kDa, 4.683
Gene length, protein length 744 bp, 248 aa
Immediate neighbours spoIISB, pit
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SpoIISA context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

sporulation proteins, toxins, antitoxins and immunity against toxins, membrane proteins

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

  • Locus tag: BSU12830

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: three putative membrane-spanning segments and a cytoplasmic domain
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • levels increase at the point of entry into stationary phase of cell cultures grown in sporulation-inducing medium, this is independent of Spo0A PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Patrik Florek, Vladimir M Levdikov, Elena Blagova, Andrey A Lebedev, Rostislav Škrabana, Stanislava Resetárová, Pamela Pavelcíková, Imrich Barak, Anthony J Wilkinson
The structure and interactions of SpoIISA and SpoIISB, a toxin-antitoxin system in Bacillus subtilis.
J Biol Chem: 2011, 286(8);6808-19
[PubMed:21147767] [WorldCat.org] [DOI] (I p)

Patrik Florek, Katarína Muchová, Pamela Pavelcíková, Imrich Barák
Expression of functional Bacillus SpoIISAB toxin-antitoxin modules in Escherichia coli.
FEMS Microbiol Lett: 2008, 278(2);177-84
[PubMed:18096016] [WorldCat.org] [DOI] (P p)

E Adler, I Barák, P Stragier
Bacillus subtilis locus encoding a killer protein and its antidote.
J Bacteriol: 2001, 183(12);3574-81
[PubMed:11371520] [WorldCat.org] [DOI] (P p)

Additional Publications: PubMed