PstBA

• Description: phosphate ABC transporter (ATP-binding protein)
  
  

Gene name	pstBA
Synonyms	pstB, yqgJ, yzmE
Essential	no
Product	phosphate ABC transporter (ATP-binding protein)
Function	high-affinity phosphate uptake
Interactions involving this protein in SubtInteract: PstBA
MW, pI	29 kDa, 7.897
Gene length, protein length	807 bp, 269 aa
Immediate neighbours	pstBB, pstA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

ABC transporters, phosphate metabolism, membrane proteins

This gene is a member of the following regulons

PhoP regulon

The gene

Basic information

• Locus tag: BSU24960

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + H₂O + phosphate(Out) = ADP + phosphate + phosphate(In) (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: (PstBA-PstBB)-(PstA-PstC)-PstS PubMed

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P46341

• KEGG entry: [2]

• E.C. number: 3.6.3.27

Expression and regulation

• Operon: pstS-pstC-pstA-pstBA-pstBB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions of phosphate limitation (5000-fold induced) (PhoP) PubMed

• Regulatory mechanism:
  • PhoP-P: transcription activation PubMed

• Additional information:
  • mRNA processing between pstS and pstC to maintain higher concentrations of PstS relative to other components of the ABC transporter PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Marion Hulett, University of Illinois at Chicago, USA Homepage