NrdE
- Description: ribonucleoside-diphosphate reductase (major subunit), control of DNA/cell mass ratio
Gene name | nrdE |
Synonyms | |
Essential | yes PubMed |
Product | ribonucleoside-diphosphate reductase (major subunit) |
Function | synthesis of deoxyribonucleoside triphosphates |
Interactions involving this protein in SubtInteract: NrdE | |
Metabolic function and regulation of this protein in SubtiPathways: Purine salvage, Nucleotides (regulation) | |
MW, pI | 80 kDa, 5.931 |
Gene length, protein length | 2100 bp, 700 aa |
Immediate neighbours | nrdI, nrdF |
Gene sequence (+200bp) | Protein sequence |
Genetic context This image was kindly provided by SubtiList
|
Contents
Categories containing this gene/protein
biosynthesis/ acquisition of nucleotides, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU17380
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin (according to Swiss-Prot)
- Protein family: ribonucleoside diphosphate reductase large chain family (according to Swiss-Prot)
- Paralogous protein(s): YosN
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure:
- UniProt: P50620
- KEGG entry: [3]
- E.C. number:
Additional information
- subject to Clp-dependent proteolysis upon glucose starvation PubMed
Expression and regulation
- Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Elisabeth Härtig, Braunschweig, Germany Homepage
Your additional remarks
References
Reviews
John Herrick, Bianca Sclavi
Ribonucleotide reductase and the regulation of DNA replication: an old story and an ancient heritage.
Mol Microbiol: 2007, 63(1);22-34
[PubMed:17229208]
[WorldCat.org]
[DOI]
(P p)
Original Publications
Additional publications: PubMed
Elisabeth Härtig, Anja Hartmann, Manuela Schätzle, Alessandra M Albertini, Dieter Jahn
The Bacillus subtilis nrdEF genes, encoding a class Ib ribonucleotide reductase, are essential for aerobic and anaerobic growth.
Appl Environ Microbiol: 2006, 72(8);5260-5
[PubMed:16885274]
[WorldCat.org]
[DOI]
(P p)
C Scotti, A Valbuzzi, M Perego, A Galizzi, A M Albertini
The Bacillus subtilis genes for ribonucleotide reductase are similar to the genes for the second class I NrdE/NrdF enzymes of Enterobacteriaceae.
Microbiology (Reading): 1996, 142 ( Pt 11);2995-3004
[PubMed:8969495]
[WorldCat.org]
[DOI]
(P p)