YorK

• Description: single-strand DNA-specific exonuclease
  
  

Gene name	yorK
Synonyms
Essential	no
Product	single-strand DNA-specific exonuclease
Function	unknown
MW, pI	65 kDa, 6.562
Gene length, protein length	1728 bp, 576 aa
Immediate neighbours	yorL, yorJ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

DNA repair/ recombination, SP-beta prophage, phosphoproteins

The gene

Basic information

• Locus tag: BSU20350

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: RecJ family

• Paralogous protein(s): YrvE

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Tyr-473 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O31903

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP895 (recJ::kan), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

V A Sutera, E S Han, L A Rajman, S T Lovett
Mutational analysis of the RecJ exonuclease of Escherichia coli: identification of phosphoesterase motifs.
J Bacteriol: 1999, 181(19);6098-102
[PubMed:10498723] [WorldCat.org] [DOI] (P p)