FrlM

• Description: aminosugar ABC transporter (permease)
  
  

Gene name	frlM
Synonyms	yurM
Essential	no
Product	aminosugar ABC transporter (permease)
Function	uptake of sugar amines
Metabolic function and regulation of this protein in SubtiPathways: Alternative nitrogen sources
MW, pI	34 kDa, 7.114
Gene length, protein length	900 bp, 300 aa
Immediate neighbours	frlD, frlN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

ABC transporters, utilization of specific carbon sources, utilization of nitrogen sources other than amino acids, membrane proteins

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

• Locus tag: BSU32580

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: MalFG subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: YurJ-(FrlM-FrlN)-FrlO PubMed

• Localization: cell membrane PubMed

Database entries

• Structure:

• UniProt: O32154

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:
  • yurR-yurQ-frlB-frlO-frlN-frlM-frlD PubMed
  • frlB-frlO-frlN-frlM-frlD PubMed

• Sigma factor:

• Regulation:
  • frlB: repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • frlB: induced in the presence of fructosamine (FrlR) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed1, PubMed2
  • FrlR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Veronika Maria Deppe, Johannes Bongaerts, Timothy O'Connell, Karl-Heinz Maurer, Friedhelm Meinhardt
Enzymatic deglycation of Amadori products in bacteria: mechanisms, occurrence and physiological functions.
Appl Microbiol Biotechnol: 2011, 90(2);399-406
[PubMed:21347729] [WorldCat.org] [DOI] (I p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)