HrcA

• Description: transcriptional repressor of class I heat-shock genes
  
  

Gene name	hrcA
Synonyms	yqxE, yqeS
Essential	no
Product	transcriptional repressor
Function	regulation of chaperone gene expression
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	38 kDa, 6.079
Gene length, protein length	1029 bp, 343 aa
Immediate neighbours	grpE, hemN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU25490

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

transcription factors and their control, heat shock proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transcription repressor of the hrcA-grpE-dnaK-dnaJ and groES-groEL operons (at low/ normal temperatures)

• Protein family: hrcA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): GroEL acts as co-repressor

• Effectors of protein activity:

• Interactions: GroEL-HrcA

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P25499

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: hrcA-grpE-dnaK-dnaJ-yqeT-yqeU-yqeV PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by heat shock (HrcA) PubMed

• Regulatory mechanism:
  • HrcA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Wolfgang Schumann, Bayreuth University, Germany Homepage

Thomas Wiegert, University of Bayreuth, Germany Homepage