PbpF

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Gene name pbpF
Synonyms ponA
Essential no
Product penicillin-binding protein 2C
Function bifunctional glucosyltransferase/ transpeptidase
MW, pI 79 kDa, 7.078
Gene length, protein length 2142 bp, 714 aa
Immediate neighbours yhgC, hemE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PbpF context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU10110

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

Categories containing this gene/protein

cell wall synthesis, sporulation proteins, membrane proteins

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot), prespore PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: expressed in mother cell and prespore PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Dirk-Jan Scheffers
Dynamic localization of penicillin-binding proteins during spore development in Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 3);999-1012
[PubMed:15758244] [WorldCat.org] [DOI] (P p)

D L Popham, P Setlow
Phenotypes of Bacillus subtilis mutants lacking multiple class A high-molecular-weight penicillin-binding proteins.
J Bacteriol: 1996, 178(7);2079-85
[PubMed:8606187] [WorldCat.org] [DOI] (P p)

D L Popham, P Setlow
Cloning, nucleotide sequence, and regulation of the Bacillus subtilis pbpF gene, which codes for a putative class A high-molecular-weight penicillin-binding protein.
J Bacteriol: 1993, 175(15);4870-6
[PubMed:8335642] [WorldCat.org] [DOI] (P p)

M Hansson, L Hederstedt
Cloning and characterization of the Bacillus subtilis hemEHY gene cluster, which encodes protoheme IX biosynthetic enzymes.
J Bacteriol: 1992, 174(24);8081-93
[PubMed:1459957] [WorldCat.org] [DOI] (P p)