HxlB

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  • Description: 6-phospho-3-hexuloisomerase

Gene name hxlB
Synonyms yckF
Essential no
Product 6-phospho-3-hexuloisomerase
Function ribulose monophosphate pathway for formaldehyde fixation
MW, pI 19 kDa, 5.791
Gene length, protein length 555 bp, 185 aa
Immediate neighbours tlpC, hxlA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
HxlB context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU03450

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

Categories containing this gene/protein

resistance against oxidative and electrophile stress

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-arabino-hex-3-ulose 6-phosphate = D-fructose 6-phosphate (according to Swiss-Prot)
  • Protein family: PHI subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: induction by formaldehyde (HxlR) PubMed
  • Regulatory mechanism: transcription activation by HxlR PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

E J Taylor, S J Charnock, J Colby, G J Davies, G W Black
Cloning, purification and characterization of the 6-phospho-3-hexulose isomerase YckF from Bacillus subtilis.
Acta Crystallogr D Biol Crystallogr: 2001, 57(Pt 8);1138-40
[PubMed:11468398] [WorldCat.org] [DOI] (P p)

H Yasueda, Y Kawahara, S Sugimoto
Bacillus subtilis yckG and yckF encode two key enzymes of the ribulose monophosphate pathway used by methylotrophs, and yckH is required for their expression.
J Bacteriol: 1999, 181(23);7154-60
[PubMed:10572115] [WorldCat.org] [DOI] (P p)