NrgB

• Description: write here
  
  

Gene name	nrgB
Synonyms
Essential
Product	nitrogen-regulated PII-like protein
Function
MW, pI	12 kDa, 8.825
Gene length, protein length	348 bp, 116 aa
Immediate neighbours
Gene sequence (+200bp)	Protein sequence
Genetic context

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: NrgA-NrgB PubMed, TnrA-NrgB PubMed

• Localization: membrane

Database entries

• Structure:

• Swiss prot entry:

• KEGG entry:

• E.C. number:

Expression and regulation

• Operon: nrgA-nrgB

• Sigma factor:

• Regulation: induced by ammonium limitation

• Regulatory mechanism: transcription activation by TnrA

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: available in Jörg Stülke lab

Labs working on this gene/protein

Susan Fisher, Boston, USA homepage

References

1. Detsch, C. & Stülke, J. (2003) Ammonium utilization in Bacillus subtilis: transport and regulatory functions of NrgA and NrgB. Microbiology 149: 3289-3297. PubMed
2. Heinrich, A., Woyda, K., Brauburger, K., Meiss, G., Detsch, C., Stülke, J. & Forchhammer, K. (2006) Interaction of the membrane-bound GlnK-AmtB complex with the master regulator of nitrogen metabolism TnrA in Bacillus subtilis. J. Biol. Chem. 281: 34909-34917. PubMed
3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed