YtsJ

• Description: malic enzyme
  
  

Gene name	ytsJ
Synonyms
Essential	no
Product	NAD-dependent malate dehydrogenase
Function	malate utilization
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	43 kDa, 5.046
Gene length, protein length	1230 bp, 410 aa
Immediate neighbours	accD, dnaE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU29220

Phenotypes of a mutant

Poor growth with malate as single carbon source PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-malate + NAD⁺ = pyruvate + CO₂ + NADH (according to Swiss-Prot) malate <--> pyruvate

• Protein family: malic enzymes family (according to Swiss-Prot)

• Paralogous protein(s): MleA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: Cytoplasm (Homogeneous) PubMed cytoplasm protein PubMed

Database entries

• Structure: 1WW8 (from Pyrococcus horikoshii, 46% identity, 63% similarity)

• UniProt: O34962

• KEGG entry: [3]

• E.C. number: 1.1.1.38

Expression and regulation

• Operon: ytsJ PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP612 (spc), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• FLAG-tag construct: GP1032 (spc, based on pGP1331), available in the Stülke lab

• Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

References

Guillaume Lerondel, Thierry Doan, Nicola Zamboni, Uwe Sauer, Stéphane Aymerich
YtsJ has the major physiological role of the four paralogous malic enzyme isoforms in Bacillus subtilis.
J Bacteriol: 2006, 188(13);4727-36
[PubMed:16788182] [WorldCat.org] [DOI] (P p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

Thierry Doan, Pascale Servant, Shigeo Tojo, Hirotake Yamaguchi, Guillaume Lerondel, Ken-Ichi Yoshida, Yasutaro Fujita, Stéphane Aymerich
The Bacillus subtilis ywkA gene encodes a malic enzyme and its transcription is activated by the YufL/YufM two-component system in response to malate.
Microbiology (Reading): 2003, 149(Pt 9);2331-2343
[PubMed:12949160] [WorldCat.org] [DOI] (P p)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)