YclK

• Description: two-component sensor kinase
  
  

Gene name	yclK
Synonyms
Essential	no
Product	two-component sensor kinase
Function	unknown
MW, pI	54 kDa, 6.152
Gene length, protein length	1419 bp, 473 aa
Immediate neighbours	yclJ, rapC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU03760

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of YclJ

• Protein family:

• Paralogous protein(s): CssS, YkoH, KinE, YvrG

Extended information on the protein

• Kinetic information:

• Domains: two transmembrane segments, C-terminal histidine phosphotransferase domain

• Modification: autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity:

• Interactions: YclK-YclJ (putative)

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P94414

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yclJ-yclK PubMed

• Sigma factor:

• Regulation:
  • expressed under anaerobic conditions (ResD) PubMed

• Regulatory mechanism:
  • ResD: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Elisabeth Härtig, Hao Geng, Anja Hartmann, Angela Hubacek, Richard Münch, Rick W Ye, Dieter Jahn, Michiko M Nakano
Bacillus subtilis ResD induces expression of the potential regulatory genes yclJK upon oxygen limitation.
J Bacteriol: 2004, 186(19);6477-84
[PubMed:15375128] [WorldCat.org] [DOI] (P p)

C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672] [WorldCat.org] [DOI] (P p)