YaaJ

• Description: tRNA specific adenosine deaminase
  
  

Gene name	yaaJ
Synonyms
Essential	no
Product	tRNA specific adenosine deaminase
Function	tRNA modification
MW, pI	17 kDa, 5.636
Gene length, protein length	483 bp, 161 aa
Immediate neighbours	yaaI, scr
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU00180

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: cytidine and deoxycytidylate deaminase family (according to Swiss-Prot)

• Paralogous protein(s):

• The corresponding gene in E. coli: EcoliWiki

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2B3J (the protein of Staphylococcus aureus, complex with RNA) PubMed

• UniProt: P21335

• KEGG entry: [3]

• E.C. number: 3.5.4.-

Expression and regulation

• Operon: yaaJ-scr

• Sigma factor: SigA

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Takashi Inaoka, Takenori Satomura, Yasutaro Fujita, Kozo Ochi
Novel gene regulation mediated by overproduction of secondary metabolite neotrehalosadiamine in Bacillus subtilis.
FEMS Microbiol Lett: 2009, 291(2);151-6
[PubMed:19087206] [WorldCat.org] [DOI] (I p)

Heather C Losey, Alexander J Ruthenburg, Gregory L Verdine
Crystal structure of Staphylococcus aureus tRNA adenosine deaminase TadA in complex with RNA.
Nat Struct Mol Biol: 2006, 13(2);153-9
[PubMed:16415880] [WorldCat.org] [DOI] (P p)

Jeannette Wolf, André P Gerber, Walter Keller
tadA, an essential tRNA-specific adenosine deaminase from Escherichia coli.
EMBO J: 2002, 21(14);3841-51
[PubMed:12110595] [WorldCat.org] [DOI] (P p)