Sda

• Description: developmental checkpoint protein, inhibits the autokinase activity of KinA and KinB, control of the phosphorelay

Gene name	sda
Synonyms
Essential	no
Product	developmental checkpoint protein
Function	mediates a developmental checkpoint coupling initiation of sporulation (phosphorylation of Spo0A) to the function of replication initiation proteins
Function and regulation of this protein in SubtiPathways: Phosphorelay
MW, pI	6 kDa, 5.605
Gene length, protein length	156 bp, 52 aa
Immediate neighbours	yloV, yqeF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU25690

Phenotypes of a mutant

• sporulates at a higher frequency
• will sporulate in the presence of replication stress

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Sda is a checkpoint protein that is upregulated in response to replication stress PubMed. Sda inhibits the autokinase activity of KinA (and likely KinB as well). This in its turn results in reduced levels of Spo0A~P. Thus, replication stressed cells will not initiate sporulation.

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: KinB-Sda PubMed, KinA-Sda PubMed

• Localization:

Database entries

• Structure: 3D36 (from Geobacillus stearothermophilus, complex with KinB) PubMed, 1PV0

• UniProt: Q7WY62

• KEGG entry: [3]

• E.C. number:

Additional information

Sda is rapidly turned over by the ClpP/X system because of some uncharged residues at its C-terminus PubMed.

Expression and regulation

• Operon: sda PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by DNA damage (LexA) PubMed

• Regulatory mechanism:
  • probably activated by DnaA under replication stress PubMed
  • LexA: transcription repression PubMed

• Additional information: Sda is rapidly turned over by the ClpP/X system because of some uncharged residues at its C-terminus PubMed.

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

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