MenF

• Description: menaquinone-specific isochorismate synthase
  
  

Gene name	menF
Synonyms	icsM, entC
Essential	no
Product	menaquinone-specific isochorismate synthase
Function	biosynthesis of menaquinone
Metabolic function and regulation of this protein in SubtiPathways: Phe, Tyr, Trp, Menaquinone
MW, pI	52 kDa, 4.706
Gene length, protein length	1413 bp, 471 aa
Immediate neighbours	menD, yteA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU30830

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Chorismate = isochorismate (according to Swiss-Prot)

• Protein family: isochorismate synthase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3BZM (from Escherichia coli, 33% identity, 51% similarity) PubMed

• UniProt: P23973

• KEGG entry: [3]

• E.C. number: 5.4.4.2

Expression and regulation

• Operon: menF-menD-ytxM-menB-menE-menC PubMed

• Sigma factor: SigA: PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)

X Qin, H W Taber
Transcriptional regulation of the Bacillus subtilis menp1 promoter.
J Bacteriol: 1996, 178(3);705-13
[PubMed:8550504] [WorldCat.org] [DOI] (P p)

J R Driscoll, H W Taber
Sequence organization and regulation of the Bacillus subtilis menBE operon.
J Bacteriol: 1992, 174(15);5063-71
[PubMed:1629163] [WorldCat.org] [DOI] (P p)