CcpN
- Description: transcriptional repressor of gluconeogenetic genes and of sr1. repression in the presence of glucose
| Gene name | ccpN |
| Synonyms | yqzB |
| Essential | no |
| Product | transcriptional regulator |
| Function | repressor of genes involved in gluconeogenesis (gapB, pckA) and of sr1 |
| Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate, Cys, Met & Sulfate assimilation, Central C-metabolism | |
| MW, pI | 23.4 kDa, 7.22 |
| Gene length, protein length | 636 bp, 212 amino acids |
| Immediate neighbours | yqfL, glyS |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU25250
Phenotypes of a mutant
Impaired growth on glucose due to re-routing of carbon from glycolysis to the pentose phosphate pathway PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: transcription repression of the gapB, pckA, and sr1 genes in the presence of glucose PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure:
- UniProt: O34994
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation: constitutively expressed PubMed
- Additional information:
- the intracellular concentration of CcpN is about 4 myM (according to PubMed).
Biological materials
- Mutant: DB104 ccpN::cat, available in Sabine Brantl lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- Antibody: available in Sabine Brantl lab
Labs working on this gene/protein
Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
Sabine Brantl, Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany homepage
Uwe Sauer, ETH Zürich, Switzerland homepage
Your additional remarks
References
Reviews
Sabine Brantl, Andreas Licht
Characterisation of Bacillus subtilis transcriptional regulators involved in metabolic processes.
Curr Protein Pept Sci: 2010, 11(4);274-91
[PubMed:20408793]
[WorldCat.org]
[DOI]
(I p)
Original Publications
Rita A Eckart, Sabine Brantl, Andreas Licht
Search for additional targets of the transcriptional regulator CcpN from Bacillus subtilis.
FEMS Microbiol Lett: 2009, 299(2);223-31
[PubMed:19732150]
[WorldCat.org]
[DOI]
(I p)
Andreas Licht, Sabine Brantl
The transcriptional repressor CcpN from Bacillus subtilis uses different repression mechanisms at different promoters.
J Biol Chem: 2009, 284(44);30032-8
[PubMed:19726675]
[WorldCat.org]
[DOI]
(I p)
Simon Tännler, Eliane Fischer, Dominique Le Coq, Thierry Doan, Emmanuel Jamet, Uwe Sauer, Stéphane Aymerich
CcpN controls central carbon fluxes in Bacillus subtilis.
J Bacteriol: 2008, 190(18);6178-87
[PubMed:18586936]
[WorldCat.org]
[DOI]
(I p)
Andreas Licht, Ralph Golbik, Sabine Brantl
Identification of ligands affecting the activity of the transcriptional repressor CcpN from Bacillus subtilis.
J Mol Biol: 2008, 380(1);17-30
[PubMed:18511073]
[WorldCat.org]
[DOI]
(I p)
Andreas Licht, Sabine Brantl
Transcriptional repressor CcpN from Bacillus subtilis compensates asymmetric contact distribution by cooperative binding.
J Mol Biol: 2006, 364(3);434-48
[PubMed:17011578]
[WorldCat.org]
[DOI]
(P p)
Andreas Licht, Sven Preis, Sabine Brantl
Implication of CcpN in the regulation of a novel untranslated RNA (SR1) in Bacillus subtilis.
Mol Microbiol: 2005, 58(1);189-206
[PubMed:16164558]
[WorldCat.org]
[DOI]
(P p)
Pascale Servant, Dominique Le Coq, Stéphane Aymerich
CcpN (YqzB), a novel regulator for CcpA-independent catabolite repression of Bacillus subtilis gluconeogenic genes.
Mol Microbiol: 2005, 55(5);1435-51
[PubMed:15720552]
[WorldCat.org]
[DOI]
(P p)
